Rat spinal cords control neural function in biobots
Source: https://neurosciencenews.com/rat-spinal-cord-biobots-16266/
Summary: Researchers combined intact neurons from a rat’s spinal cord with a tissue-engineered 3D muscle system, creating a biohybrid robot, or biobot. After culturing the system for seven days, the motor neurons from the spinal cord produced electrical activity, causing contractions in the artificial muscles and mimicking the behavior of the peripheral nervous system. The findings could have positive implications for the treatment of neurodegenerative disorders that affect motor control.
Source: AIP
Biological robots, or biobots, draw inspiration from natural systems to mimic the motions of organisms, such as swimming or jumping. Improvements to biobots to better replicate complex motor behaviors can lead to exciting biorobotic engineering applications to help solve real world challenges. However, this requires the creation of biohybrid robots — biobots made up of both organic and artificial materials — which is a challenge.
Researchers from the University of Illinois at Urbana-Champaign combined an intact rat spinal cord with a tissue-engineered, 3D muscle system. They describe the novel biohybrid system in the journal APL Bioengineering, from AIP Publishing.
After culturing the system for seven days, the researchers found that the motor neurons from the spinal cord begin to produce electrical activity that causes contraction in the artificial muscles, mirroring the behavior of the peripheral nervous system.
“When we looked more deeply at how the neuron-muscle interface developed, we were very excited to observe many similarities between our tissue-engineered spinobot and in vivo development,” said author Collin Kaufman, a neuroscience graduate student at UIUC.
Kaufman said this result indicates the explanted spinal cord is a viable mechanism for controlling muscular behaviors, even when removed from its natural environment.
They further tested this by varying the concentration of neurotransmitters in the system. When additional neurotransmitters are present, the contractions are more patterned and consistent, and when they are blocked, the twitching decreases.
Because studying the peripheral nervous system can be so difficult, the ability to observe it externally — as demonstrated in the present study — can lead to great strides in medicine.
One potential example is in Lou Gehrig’s disease, also known as amyotrophic lateral sclerosis, in which the death of neurons results in the eventual loss of motor function. By developing an external peripheral nervous system, researchers can study ALS with ease of access to the impacted components in real time.
“The next steps to studying such a disease are surprisingly close,” Kaufman said. “By replacing the muscle, the spinal cord, or any combination of the two tissues with an ALS mutant model, researchers would be able to study how diseased neurons interact with nearby muscles.”
In addition, hybrid biobots can be used as surgical training tools, allowing medical students to perform practice surgery on real biological tissue.
“The future applications of this technology are only beginning to be understood, and we expect many great things from this area in the next few years,” said Martha Gillette, professor of cell and developmental biology at UIUC.
About this neuroscience research article
Source:
AIP
Media Contacts:
Larry Frum – AIP
Image Source:
The image is credited to Collin D. Kaufman.
Original Research: Open access
“Emergence of functional neuromuscular junctions in an engineered, multicellular spinal cord-muscle bioactuator”. by C. D. Kaufman et al.
Cell Reports doi:10.1063/1.5121440
Abstract
Emergence of functional neuromuscular junctions in an engineered, multicellular spinal cord-muscle bioactuator
Three-dimensional (3D) biomimetic systems hold great promise for the study of biological systems in vitro as well as for the development and testing of pharmaceuticals. Here, we test the hypothesis that an intact segment of lumbar rat spinal cord will form functional neuromuscular junctions (NMJs) with engineered, 3D muscle tissue, mimicking the partial development of the peripheral nervous system (PNS). Muscle tissues are grown on a 3D-printed polyethylene glycol (PEG) skeleton where deflection of the backbone due to muscle contraction causes the displacement of the pillar-like “feet.” We show that spinal cord explants extend a robust and complex arbor of motor neurons and glia in vitro. We then engineered a “spinobot” by innervating the muscle tissue with an intact segment of lumbar spinal cord that houses the hindlimb locomotor central pattern generator (CPG). Within 7 days of the spinal cord being introduced to the muscle tissue, functional neuromuscular junctions (NMJs) are formed, resulting in the development of an early PNS in vitro. The newly innervated muscles exhibit spontaneous contractions as measured by the displacement of pillars on the PEG skeleton. Upon chemical excitation, the spinal cord-muscle system initiated muscular twitches with a consistent frequency pattern. These sequences of contraction/relaxation suggest the action of a spinal CPG. Chemical inhibition with a blocker of neuronal glutamate receptors effectively blocked contractions. Overall, these data demonstrate that a rat spinal cord is capable of forming functional neuromuscular junctions ex vivo with an engineered muscle tissue at an ontogenetically similar timescale.
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